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| Registros recuperados: 29 | |
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| Fonseca,María Isabel; Tejerina,Marcos Raúl; Sawostjanik-Afanasiuk,Silvana Soledad; Giorgio,Ernesto Martin; Barchuk,Mónica Lucrecia; Zapata,Pedro Darío; Villalba,Laura Lidia. |
| Abstract Oxidative enzymes secreted by white rot fungi can be applied in several technological processes within the paper industry, biofuel production and bioremediation. The discovery of native strains from the biodiverse Misiones (Argentina) forest can provide useful enzymes for biotechnological purposes. In this work, we evaluated the laccase and manganese peroxidase secretion abilities of four newly discovered strains of Trametes sp. that are native to Misiones. In addition, the copper response and optimal pH and temperature for laccase activity in culture supernatants were determined.The selected strains produced variable amounts of laccase and MnP; when Cu2+ was added, both enzymes were significantly increased. Zymograms showed that two isoenzymes... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: White rot fungi; Trametes; Laccase; Manganese peroxidase; Cu2+ response. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200287 |
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| Levin,L.; Ramos,A .M.; Parisi,M .; Gally,M.. |
| Colletotrichum truncatum is the most common pathogen fungus associated with soybean anthracnose. Although the lignin-degrading enzyme laccase has been implicated in pathogenicity of a wide range of plant pathogenic fungi, its biological role in the Colletotrichum -soybean disease system is unknown. The extent of the infection in our country led us to examine laccase production in Argentinean Colletotrichum strains isolated from diseased soybean plants from different geographic locations. Ten strains (eight of them identified as C. truncatum) , were screened for in vitro laccase production. Only six of the isolates, all of them C. truncatum , produced laccase activity when cultured on a defined medium based on pectin and asparagine as carbon and nitrogen... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Anthracnose; Colletotrichum; Laccase; SDS-PAGE; Soybean. |
| Ano: 2007 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-23722007000100010 |
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| Cavallazzi,José Renato P.; Kasuya,Catarina M.; Soares,Marcos A.. |
| Laccases are enzymes involved in lignin degradation and are produced by various organisms. Due to their low substrate specificity their potential to be used in biotechnological applications has received attention. The addition of laccase inducers to the culture medium of microorganisms can enhance laccase production and facilitate its purification and utilization. The aim of this study was to investigate the effect of some compounds as laccase inducers in cultures of Lentinula edodes (shiitake). First, it was selected a culture medium suitable for laccase production by shiitake using two levels of N (2.6 and 26 mM) and seven levels of Cu (0, 50, 100, 150, 200, 250 and 300 µM). The medium with 2.6 mM N and 250 µM Cu was found to provide the highest laccase... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Laccase; Shiitake; Laccase inducers. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000400015 |
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| Camassola,Marli; Rosa,Letícia O. da; Calloni,Raquel; Gaio,Tamara A.; Dillon,Aldo J.P.. |
| Pleurotus species secrete phenol oxidase enzymes: laccase (Lcc) and manganese peroxidase (MnP). New genotypes of these species show potential to be used in processes aiming at the degradation of phenolic compounds, polycyclic aromatic hydrocarbons and dyes. Hence, a screening of some strains of Pleurotus towards Lcc and MnP production was performed in this work. Ten strains were grown through solid-state fermentation on a medium based on Pinus spp. sawdust, wheat bran and calcium carbonate. High Lcc and MnP activities were found with these strains. Highest Lcc activity, 741 ± 245 U gdm-1 of solid state-cultivation medium, was detected on strain IB11 after 32 days, while the highest MnP activity occurred with strains IB05, IB09, and IB11 (5,333 ± 357; 4,701... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pleurotus; Laccase; Manganese peroxidase; Mushroom production; Biotechnological applications. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100031 |
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| Spezzia Mazzocco, Teresita. |
| Quince cepas de hongos comestibles pertenecientes a los géneros Pleurotus, Ganoderma, Lentinula y Coprinopsis, fueron evaluadas en cuanto a su actividad enzimática utilizando el medio de cultivo agar con extracto de malta (EMA), adicionado con ácido 2,2'–azino–bis–(3–etillbenzotiazolin–6–sulfónico) (ABTS), 2,6-dimetoxyphenol (DMP) y guayacol. Se seleccionaron nueve cepas para determinar cuantitativamente la actividad de lacasas. Esta evaluación se realizó con el medio de cultivo de extracto de malta líquido (EMB) suplementado con 0.1 mM de sulfato de cobre. Se demostró que las cepas CP-50 de Pleurotus ostreatus y CP-267 de P. pulmonarius son altamente productoras de actividad de lacasas, obteniéndose 4,000 U/ml y 2,500 U/ml, respectivamente. Estas cepas... |
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Palavras-chave: Antraceno; Biorremediación; Hongos comestibles; Lacasas; EDAR; Estrategias para el Desarrollo Agrícola Regional; Doctorado; Anthracene; Bioremediation; Laccase; Mushrooms. |
| Ano: 2012 |
URL: http://hdl.handle.net/10521/1826 |
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| Serna C,Liliana; Cuesta A,Fernando L. |
| Contamination is defined as the presence of pollution agents in an ecosystem reaching an abnormal concentration level and causing adverse effects. Due to the important environmental problems that these cause in the food chain, diverse methods have been formulated to minimize those effects; among these enzymatic methods associated to microorganisms or other biological agents are used, and systems that allow to metabolize pollutants in an effective and practical way. The combined use of these systems appears to be the most appropriated option to eliminate pollution agents at a reasonable energy cost which allows their use in the industry. The present review identifies the adequate process conditions for technological implementation of biofilm-type membrane... |
| Tipo: Journal article |
Palavras-chave: Laccase; Biofilm; Membrane system; Halogen compound. |
| Ano: 2008 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392008000400010 |
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| Reena,; Dhall,Purnima; Kumar,Rita; Kumar,Anil. |
| Present study reports the validation (oxidation) of computationally predicted oxidation of xenobiotic contaminants by commercially available pure laccase from Trametes versicolor. Selected contaminants were predicted as potential targets for laccase oxidation by using in-silico docking tool. The oxidation by laccase was measured by change in absorbance at specific λ max of each compound. Sinapic acid and tyrosine were taken as positive and negative controls, respectively. Oxidation was observed in m-chlorophenol, 2,4 di-chlorophenol, 2,4,6 tri-chlorophenol, captan, atrazine and thiodicarb, except malathion, which showed no activity. It could be speculated that the predicted substrates showing oxidation shared homology at structural and chemical level with... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biodegradation; In silico screening; Laccase; Xenobiotics. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000500803 |
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| Singh,Gursharan; Batish,Mona; Sharma,Prince; Capalash,Neena. |
| Various genotoxic textile dyes, xenobiotics, substrates (10 µM) and agrochemicals (100 µg/ml) were tested for enhancement of alkalophilic laccase activity inγ-proteobacterium JB. Neutral Red, Indigo Carmine, Naphthol Base Bordears and Sulphast Ruby dyes increased the activity by 3.7, 2.7, 2.6 and 2.3 fold respectively. Xenobiotics/substrates like p-toluidine, 8-hydroxyquinoline and anthracine increased it by 3.4, 2.8 and 2.3 fold respectively. Atrazine and trycyclozole pesticides enhanced the activityby 1.95 and 1.5 fold respectively. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Γ-proteobacterium; Laccase; Xenobiotics; Dyes; Agrochemicals. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100004 |
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| Registros recuperados: 29 | |
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